Publications in VIVO

Co-author Network

Falke, Joseph

Professor

Positions

Professor, Biochemistry

Research Areas

Research

research overview

Dr. Falke's laboratory investigates the molecular mechanisms of membrane-based lipid signaling pathways that enable eukaryotic cells to (i) follow chemical trails in innate immunity and development, (ii) generate growth signals in normal and unregulated cell growth, (iii) engulf, or phagocytose, extracellular pathogens or damaged tissue in innate immunity, and (iv) engulf, or autophagocytose, intracellular pathogens and aging components. The work is revealing fundamental insights into the molecular nature of lipid signaling on membrane surfaces important in signaling biology, immunology, cancer biology and medicine. The lab is also developing tools widely useful in biochemistry and biophysics.

keywords

biochemistry and biophysics of cellular signaling pathways and membrane proteins, macrophage chemotaxis and phagocytosis, cancer, inflammation, innate immunity

Publications

selected publications

conference proceeding
- PDK1 Forms Homodimers on Supported Lipid Bilayers in a PIP3 Dependent Manner. Biophysical Journal. 287A-288A. 2021
- Binding of Ras to PI3K: Measuring Binding Affinity, and the Effects of Disease-Linked H-Ras Mutations on Affinity. Biophysical Journal. 282A-282A. 2020
- Direct Detection and Characterization of a Phosphoinositide Dependent Kinase-1 (PDK1) Homodimer on a Target Membrane Surface Via Single Molecule Fluorescence. Biophysical Journal. 558A-558A. 2020
- Regulatory Mechanisms of Ca2+, Receptor, Ras, and Lipid Signals that Control Actin Polymerization During Cell Migration. Biophysical Journal. 15A-16A. 2020
- Histidine Kinase CheA: You Spin Me 'Round. The FASEB Journal. 2017
- Activation of PI3K by Ras: Single Molecule Studies of the Activation Mechanism. Biophysical Journal. 393A-393A. 2014
- Single Molecule Studies of PKCα Activation Mechanism on Membrane Surfaces. Biophysical Journal. 513A-513A. 2014
- Single Molecule Studies of a Membrane-Bound, Master Kinase Circuit ... Detecting Interactions of Two Master Kinase Pairs on the Membrane, PDK1-AKT1 and PDK1-PKC Alpha. Biophysical Journal. 392A-392A. 2014
- Molecular Mechanism of an Oncogenic Mutation that Alters Membrane Targeting: Glu17Lys Modifies the PIP Lipid Specificity of AKT1 PH Domain. Biophysical Journal. 613A-613A. 2009
- Single Molecule Fluorescence Studies of Membrane Targeting Proteins: Lateral Diffusion in Supported Bilayers Reveals Additional Lipid Binding Sites. Biophysical Journal. 207A-207A. 2009
journal article
- Ras signaling mechanisms: New insights from single-molecule biophysics. Biophysical Journal. 3277-3280. 2024
- PDK1:PKCa heterodimer association-dissociation dynamics in single-molecule diffusion tracks on a target membrane. Biophysical Journal. 2301-2310. 2023
- Binding of active Ras and its mutants to the Ras binding domain of PI-3-kinase: A quantitative approach to KD measurements. Analytical Biochemistry. 2023
- Single-molecule studies reveal regulatory interactions between master kinases PDK1, AKT1, and PKC. Biophysical Journal. 5657-5673. 2021
- HPLC method to resolve, identify and quantify guanine nucleotides bound to recombinant ras GTPase. Analytical Biochemistry. 2021
- Ras-guanine nucleotide complexes: A UV spectral deconvolution method to analyze protein concentration, nucleotide stoichiometry, and purity. Analytical Biochemistry. 2021
- The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism. Biophysical Journal. 2205-2218. 2020
- Rapid exposure of macrophages to drugs resolves four classes of effects on the leading edge sensory pseudopod: Non-perturbing, adaptive, disruptive, and activating. PLoS One. 2020
- A PKC-MARCKS-PI3K regulatory module links Ca²⁺ and PIP₃ signals at the leading edge of polarized macrophages. PLoS One. 2018
- Single-Molecule Study Reveals How Receptor and Ras Synergistically Activate PI3Kα and PIP₃ Signaling. Biophysical Journal. 2396-2405. 2017
- Regulation of a Coupled MARCKS-PI3K Lipid Kinase Circuit by Calmodulin: Single-Molecule Analysis of a Membrane-Bound Signaling Module. Biochemistry. 6395-6405. 2016
- Regulation of PI3K by PKC and MARCKS: Single-Molecule Analysis of a Reconstituted Signaling Pathway. Biophysical Journal. 1811-1825. 2016
- Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update. Trends in Microbiology. 257-266. 2015
- Disulfide Trapping and Spectroscopic Studies of Bacterial Chemosensory Core Signaling Complexes: Probing Molecular Mechanisms of Complex Assembly and Receptor-Regulated On-Off Switching. Biophysical Journal. 42A-42A. 2015
- Architecture and signal transduction mechanism of the bacterial chemosensory array: Progress, controversies, and challenges. Current Opinion in Structural Biology. 85-94. 2014
- Increasing and Decreasing the Ultrastability of Bacterial Chemotaxis Core Signaling Complexes by Modifying Protein-Protein Contacts. Biochemistry. 5592-5600. 2014
- Piston versus Scissors: Chemotaxis Receptors versus Sensor His-Kinase Receptors in Two-Component Signaling Pathways. Structure. 1219-1220. 2014
- Interplay between phosphoinositide lipids and calcium signals at the leading edge of chemotaxing ameboid cells. Chemistry and Physics of Lipids. 73-79. 2014
- Interactions of Protein Kinase C-α C1A and C1B Domains with Membranes: A Combined Computational and Experimental Study. Journal of the American Chemical Society. 11757-11766. 2014
- New Insights into Bacterial Chemoreceptor Array Structure and Assembly from Electron Cryotomography. Biochemistry. 1575-1585. 2014
- Single-Molecule Studies Reveal a Hidden Key Step in the Activation Mechanism of Membrane-Bound Protein Kinase C-α. Biochemistry. 1697-1713. 2014
- Structure, Function, and On-Off Switching of a Core Unit Contact between CheA Kinase and CheW Adaptor Protein in the Bacterial Chemosensory Array: A Disulfide Mapping and Mutagenesis Study. Biochemistry. 7753-7765. 2013
- Molecular Mechanism of Membrane Binding of the GRP1 PH Domain. Journal of Molecular Biology. 3073-3090. 2013
- The PH Domain of Phosphoinositide-Dependent Kinase-1 Exhibits a Novel, Phospho-Regulated Monomer-Dimer Equilibrium with Important Implications for Kinase Domain Activation: Single-Molecule and Ensemble Studies. Biochemistry. 4820-4829. 2013
- Lateral diffusion of peripheral membrane proteins on supported lipid bilayers is controlled by the additive frictional drags of (1) bound lipids and (2) protein domains penetrating into the bilayer hydrocarbon core. Chemistry and Physics of Lipids. 67-77. 2013
- Defining a Key Receptor-CheA Kinase Contact and Elucidating Its Function in the Membrane-Bound Bacterial Chemosensory Array: A Disulfide Mapping and TAM-IDS Study. Biochemistry. 3866-3880. 2013
- The 3.2 Å Resolution Structure of a Receptor:CheA:CheW Signaling Complex Defines Overlapping Binding Sites and Key Residue Interactions within Bacterial Chemosensory Arrays. Biochemistry. 3852-3865. 2013
- Probing the Structural Origins of Unusually Strong Target Membrane Affinity of Synaptotagmin 7 C2A and C2Ab Domains. Biophysical Journal. 537A-537A. 2013
- Isolated Bacterial Chemosensory Array Possesses Quasi- and Ultrastable Components: Functional Links between Array Stability, Cooperativity, and Order. Biochemistry. 10218-10228. 2012
- Hydrophobic Contributions to the Membrane Docking of Synaptotagmin 7 C2A Domain: Mechanistic Contrast between Isoforms 1 and 7. Biochemistry. 7654-7664. 2012
- Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study. PLoS One. 2012
- Assembly of Membrane-Bound Protein Complexes: Detection and Analysis by Single Molecule Diffusion. Biochemistry. 1638-1647. 2012
- Lipid targeting domain with dual-membrane specificity that expands the diversity of intracellular targeting reactions. Proceedings of the National Academy of Sciences of USA. 1816-1817. 2012
- Analysis of Protein Complex Formation on Membrane Surfaces by Single Molecule Diffusion. Biophysical Journal. 183A-183A. 2012
- Synaptotagmin C2 Domain Membrane Targeting: Kinetic and Mechanistic Diversity Among Isoforms from Different Cell Types. Biophysical Journal. 433A-433A. 2012
- The GRP1 PH Domain, Like the AKT1 PH Domain, Possesses a Sentry Glutamate Residue Essential for Specific Targeting to Plasma Membrane PI(3,4,5)P₃. Biochemistry. 9845-9856. 2011
- WITHDRAWN: Reprint of: Purification of Proteins Using Polyhistidine Affinity Tags. Protein Expression and Purification. 2011
- New insights into protein-membrane interaction from single-molecule TIRF microscopy. ACS National Meeting Book of Abstracts. 2011
- Protein-Lipid Interactions Shaping the Electrostatic Membrane Search of a Pleckstrin Homology Domain. Biophysical Journal. 525-525. 2011
- OS-FRET: A New One-Sample Method for Improved FRET Measurements. Biochemistry. 451-457. 2011
- Single Molecule Diffusion of Membrane-Bound Proteins: Window into Lipid Contacts and Bilayer Dynamics. Biophysical Journal. 2879-2887. 2010
- Membrane Docking Geometry and Target Lipid Stoichiometry of Membrane-Bound PKCα C2 Domain: A Combined Molecular Dynamics and Experimental Study. Journal of Molecular Biology. 301-310. 2010
- EVIDENCE THAT TRICYCLIC SMALL MOLECULES MAY POSSESS TOLL-LIKE RECEPTOR AND MYELOID DIFFERENTIATION PROTEIN 2 ACTIVITY. Neuroscience. 551-563. 2010
- Evidence that opioids may have toll-like receptor 4 and MD-2 effects. Brain, Behavior, and Immunity. 83-95. 2010
- Membrane Diffusion of PH Domain-PIP3 Complexes: the Effects of Target Lipid Stoichiometry on Diffusion Constant Probed Using Single-Molecule Fluorescence Microscopy. Biophysical Journal. 86A-86A. 2010
- Second Messengers at the Leading Edge: Calcium Joins PIP3 as an Essential Signal. Biophysical Journal. 620A-620A. 2010
- Structural Analysis of the Membrane Docking Geometry of PI(3,4,5)P3-Specific GRP1-PH Domain Via Site-Directed Spin Labeling. Biophysical Journal. 399A-400A. 2010
- Engineered Socket Study of Signaling through a Four-Helix Bundle: Evidence for a Yin-Yang Mechanism in the Kinase Control Module of the Aspartate Receptor. Biochemistry. 9266-9277. 2009
- The Piston Rises Again. Structure. 1149-1151. 2009
- The Core Signaling Proteins of Bacterial Chemotaxis Assemble To Form an Ultrastable Complex. Biochemistry. 6975-6987. 2009
- Thermal Domain Motions of CheA Kinase in Solution: Disulfide Trapping Reveals the Motional Constraints Leading to Trans-autophosphorylation. Biochemistry. 3631-3644. 2009
- Molecular mechanisms of specific membrane targeting by conserved protein domains. ACS National Meeting Book of Abstracts. 2009
- Single-Molecule Fluorescence Studies of a PH Domain: New Insights into the Membrane Docking Reaction. Biophysical Journal. 566-582. 2009
- Molecular Mechanism of an Oncogenic Mutation That Alters Membrane Targeting: Glu17Lys Modifies the PIP Lipid Specificity of the AKT1 PH Domain. Biochemistry. 12260-12269. 2008
- Effect of PIP₂ binding on the membrane docking geometry of PKCα C2 domain:: An EPR site-directed spin-labeling and relaxation study. Biochemistry. 8301-8316. 2008
- COLL 85-Single molecule studies of PH and C2 domain docking to model membranes. ACS National Meeting Book of Abstracts. 2008
- Bacterial chemoreceptors: high-performance signaling in networked arrays. Trends in Biochemical Sciences. 9-19. 2008
- Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: A disulfide mapping study. Biochemistry. 13684-13695. 2007
- Ca²⁺ influx is an essential component of the positive-feed back loop that maintains leading-edge structure and activity in macrophages. Proceedings of the National Academy of Sciences of USA. 16176-16181. 2007
- Chemotaxis receptor complexes: From signaling to assembly. PLoS Computational Biology. 1385-1393. 2007
- Mechanism of specific membrane targeting by C2 domains:: Localized pools of target lipids enhance Ca²⁺ affinity. Biochemistry. 4322-4336. 2007
- Investigation of PH domain-membrane interactions using T1RFM. Biophysical Journal. 68A-68A. 2007
- Membrane Recruitment as a Cancer Mechanism: A Case Study of Akt PH Domain.. Cellscience. 25-30. 2007
- Self-induced docking site of a deeply embedded peripheral membrane protein. Biophysical Journal. 517-524. 2007
- Structural and functional studies of the membrane targeting mechanism of phosphoinositide-specifie pleckstrin homology domains. Biophysical Journal. 586A-586A. 2007
- CheA kinase of bacterial chemotaxis: Chemical mapping of four essential docking sites. Biochemistry. 8699-8711. 2006
- Investigating the intracellular targeting specificity of C2 domains. ACS National Meeting Book of Abstracts. 2006
- An Extracellular Calcium Influx Is Required for Phosphatidylinositol 3-Kinase Activity and Membrane Ruffling in Macrophages. Molecular Biology of the Cell. 2006
- Specific translocation of protein kinase Cα to the plasma membrane requires both Ca²⁺ and PIP₂ recognition by its C2 domain. Molecular Biology of the Cell. 56-66. 2006
- Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: Cysteine and disulfide scanning studies. Biochemistry. 12655-12666. 2005
- Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching. Biochemistry. 7687-7695. 2005
- Adaptation mechanism of the aspartate receptor: Electrostatics of the adaptation subdomain play a key role in modulating kinase activity. Biochemistry. 1550-1560. 2005
- Combining experimental and computational techniques to reveal bases for ionic selectivity and binding in the cPLA(2) C2 domain. Biophysical Journal. 82A-83A. 2005
- Molecular dynamics simulation of the C2 domain of cytosolic phospholipase A2. Biophysical Journal. 403A-403A. 2005
- Use of EPR power saturation toanalyze the membrane-docking geometries of peripheral proteins: A applications to C2 domains. Annual Review of Biophysics. 71-90. 2005
- Call activation of the cPLA(2) C2 domain: Ordered binding of two Ca2+ ions with positive cooperativity. Biochemistry. 16320-16328. 2004
- GRP1 pleckstrin homology domain: Activation parameters and novel search mechanism for rare target lipid. Biochemistry. 16161-16173. 2004
- Characterization of the membrane docking mechanism of the GRP1 Pleckstrin homology domain. Journal of General Physiology. 17A-17A. 2004
- Sensing chemical signals at membrane surfaces. Journal of General Physiology. 21A-21A. 2004
- Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor. Biochemistry. 1763-1770. 2004
- Chemotaxis receptors and signaling. Advances in Protein Chemistry. 393-+. 2004
- Membrane-docking loops of the cPLA2 C2 domain: Detailed structural analysis of the protein-membrane interface via site-directed spin-labeling. Biochemistry. 13227-13240. 2003
- Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling.. Biochemistry. 13227-13240. 2003
- Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation. Biochemistry. 2952-2959. 2003
- Quantitative analysis of aspartate receptor signaling complex reveals that the homogeneous two-state model is inadequate: Development of a heterogeneous two-state model. Journal of Molecular Biology. 1597-1614. 2003
- C2 domain of protein kinase Cα:: Elucidation of the membrane docking surface by site-directed fluorescence and spin labeling. Biochemistry. 1254-1265. 2003
- Aspartate receptor transmembrane signaling and kinase regulation. Biophysical Journal. 394A-394A. 2003
- C2 domains of protein kinase C isoforms α, β, and γ:: Activation parameters and calcium stoichiometries of the membrane-bound state. Biochemistry. 11411-11424. 2002
- Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling. Biochemistry. 6282-6292. 2002
- Cooperativity between bacterial chemotaxis receptors. Proceedings of the National Academy of Sciences of USA. 6530-6532. 2002
- A moving story. Science. 1480-1481. 2002
- Cation charge and size selectivity of the C2 domain of cytosolic phospholipase A(2). Biochemistry. 1109-1122. 2002
- C2 domain of cPLA(2): Structural and dynamic analysis of Ca2+ activation and membrane docking. Biophysical Journal. 58A-58A. 2002
- C2 domain of cPLA2: Structural analysis of Ca2+ activation and membrane docking. Biophysical Journal. 172A-172A. 2002
- Functional proteomics analysis of PKC calcium-activated C2 domains. Biophysical Journal. 58A-58A. 2002
- Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains.. Methods in Molecular Biology. 295-303. 2002
- Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex. Journal of General Physiology. 693-710. 2001
- Transmembrane signaling in bacterial chemoreceptors. Trends in Biochemical Sciences. 257-265. 2001
- C2 domains from different Ca²⁺ signaling pathways display functional and mechanistic diversity. Biochemistry. 3089-3100. 2001
- C2 domains of PKCa and PKCb: Functional differences. Biophysical Journal. 458A-458A. 2001
- Conformations of the membrane-docking loops in a membrane-bound C2 domain.. Biophysical Journal. 534A-534A. 2001
- Probing the mechanisms of kinase regulation and covalent adaptation in the cytoplasmic domain of the aspartate receptor. Biophysical Journal. 198A-198A. 2001
- The aspartate receptor: Mechanisms of covalent adaptation and kinase regulation. Biophysical Journal. 247A-247A. 2001
- Attractant regulation of the aspartate receptor-kinase complex: Limited cooperative interactions between receptors and effects of the receptor modification state. Biochemistry. 9486-9493. 2000
- Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors. Current Opinion in Structural Biology. 462-469. 2000
- Ca2+-activated membrane docking by the ubiquitous C2 domain: Comparison of C2 domains from different functional families.. Biophysical Journal. 69A-69A. 2000
- Purification of proteins using polyhistidine affinity tags. Methods in Enzymology. 245-254. 2000
- Signaling domain of the aspartate receptor is a helical hairpin with a localized kinase docking surface: Cysteine and disulfide scanning studies. Biochemistry. 9317-9327. 1999
- The aspartate receptor cytoplasmic domain: in situ chemical analysis of structure, mechanism and dynamics. Structure. 829-840. 1999
- Identification of a site critical for kinase regulation on the central processing unit (CPU) helix of the aspartate receptor. Biochemistry. 329-336. 1999
- Calcium-activated membrane targetting by the C2 domain.. Biophysical Journal. A135-A135. 1999
- Comparison of cardiac and skeletal troponin C. Biophysical Journal. A158-A158. 1999
- Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A2.. Biophysical Journal. A232-A232. 1999
- Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A(2). Biochemistry. 17642-17650. 1998
- The kinetic cycle of cardiac troponin C: Calcium binding and dissociation at site II trigger slow conformational rearrangements. Protein Science. 2451-2459. 1998
- Detection of a conserved alpha-helix in the kinase-docking region of the aspartate receptor by cysteine and disulfide scanning. Journal of Biological Chemistry. 25006-25014. 1998
- Cysteine and disulfide scanning reveals two amphiphilic helices in the linker region of the aspartate chemoreceptor. Biochemistry. 10746-10756. 1998
- Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A₂. Journal of Biological Chemistry. 1365-1372. 1998
- Cysteine and disulfide scanning reveals a regulatory alpha-helix in the cytoplasmic domain of the aspartate receptor. Journal of Biological Chemistry. 32878-32888. 1997
- Ca2+-signaling cycle of a membrane-docking C2 domain. Biochemistry. 12011-12018. 1997
- Optimizing the metal binding parameters of an EF-hand-like calcium chelation loop: Coordinating side chains play a more important tuning role than chelation loop flexibility. Biochemistry. 9917-9926. 1997
- Molecular tuning of an EF-hand-like calcium binding loop - Contributions of the coordinating side chain at loop position 3. Journal of General Physiology. 173-184. 1997
- Intermolecular tuning of calmodulin by target peptides and proteins: Differential effects on Ca2+ binding and implications for kinase activation. Protein Science. 794-807. 1997
- The two-component signaling pathway of bacterial chemotaxis: A molecular view of signal transduction by receptors, kinases, and adaptation enzymes. Annual Review of Cell and Developmental Biology. 457-512. 1997
- The C2 domain calcium-binding motif: Structural and functional diversity. Protein Science. 2375-2390. 1996
- Effects of protein stabilizing agents on thermal backbone motions: A disulfide trapping study. Biochemistry. 10595-10600. 1996
- Tuning the equilibrium ion affinity and selectivity of the EF-hand calcium binding motif: Substitutions at the gateway position. Biochemistry. 6697-6705. 1996
- Molecular mechanism of transmembrane signaling by the aspartate receptor: A model. Proceedings of the National Academy of Sciences of USA. 2545-2550. 1996
- Kinetic tuning of the EF-Hand calcium binding motif: The gateway residue independently adjusts (I) barrier height and (II) equilibrium. Biochemistry. 1753-1760. 1996
- Tuning ion affinity and selectivity of the EF-hand Ca2+ binding motif: Changes at the gateway position. Biophysical Journal. TUPM8-TUPM8. 1996
- Use of F-19 NMR to probe protein structure and conformational changes. Annual Review of Biophysics. 163-195. 1996
- Use of F-19 NMR to probe protein structure and conformational changes. Annual Review of Biophysics. 163-195. 1996
- LOCK ON OFF DISULFIDES IDENTIFY THE TRANSMEMBRANE SIGNALING HELIX OF THE ASPARTATE RECEPTOR. Journal of Biological Chemistry. 24043-24053. 1995
- TRANSMEMBRANE SIGNALING - A MOLECULAR VIEW OF THE ASPARTATE RECEPTOR. ACS National Meeting Book of Abstracts. 331-PHYS. 1995
- TRANSMEMBRANE SIGNALING BY THE ASPARTATE RECEPTOR - ENGINEERED DISULFIDES REVEAL STATIC REGIONS OF THE SUBUNIT INTERFACE. Biochemistry. 9722-9733. 1995
- BLAST-1995 - INTERNATIONAL-CONFERENCE ON BACTERIAL LOCOMOTION AND SIGNAL-TRANSDUCTION. Molecular Microbiology. 1037-1050. 1995
- LARGE-AMPLITUDE TWISTING MOTIONS OF AN INTERDOMAIN HINGE - A DISULFIDE TRAPPING STUDY OF THE GALACTOSE-GLUCOSE BINDING-PROTEIN. Biochemistry. 3048-3055. 1995
- MOLECULAR TUNING OF ION-BINDING TO CALCIUM SIGNALING PROTEINS. Quarterly Reviews of Biophysics. 219-290. 1994
- ATTRACTANT-INDUCED AND DISULFIDE-INDUCED CONFORMATIONAL-CHANGES IN THE LIGAND-BINDING DOMAIN OF THE CHEMOTAXIS ASPARTATE RECEPTOR - A F-19 NMR-STUDY. Biochemistry. 6100-6109. 1994
- KINETIC CONTROL OF CA(II) SIGNALING - TUNING THE ION DISSOCIATION RATES OF EF-HAND CA(II) BINDING-SITES. Proceedings of the National Academy of Sciences of USA. 6493-6497. 1993
- ACTIVATION OF THE PHOSPHOSIGNALING PROTEIN CHEY .1. ANALYSIS OF THE PHOSPHORYLATED CONFORMATION BY F-19 NMR AND PROTEIN ENGINEERING. Journal of Biological Chemistry. 13081-13088. 1993
- ACTIVATION OF THE PHOSPHOSIGNALING PROTEIN CHEY .2. ANALYSIS OF ACTIVATED MUTANTS BY F-19 NMR AND PROTEIN ENGINEERING. Journal of Biological Chemistry. 13089-13096. 1993
- NOVEL ION SPECIFICITY OF A CARBOXYLATE CLUSTER MG(II) BINDING-SITE - STRONG CHARGE SELECTIVITY AND WEAK SIZE SELECTIVITY. Biochemistry. 3363-3367. 1993
- THERMAL MOTIONS OF SURFACE ALPHA-HELICES IN THE D-GALACTOSE CHEMOSENSORY RECEPTOR - DETECTION BY DISULFIDE TRAPPING. Journal of Molecular Biology. 1219-1235. 1992
- ENGINEERING THE ION SPECIFICITY OF A PROTEIN CA(II) SITE. ACS National Meeting Book of Abstracts. 713-INOR. 1992
- F-19 NUCLEAR-MAGNETIC-RESONANCE STUDIES OF AQUEOUS AND TRANSMEMBRANE RECEPTORS - EXAMPLES FROM THE ESCHERICHIA-COLI CHEMOSENSORY PATHWAY. Biophysical Journal. 82-86. 1992
- STRUCTURE AND DYNAMICS OF ESCHERICHIA-COLI CHEMOSENSORY RECEPTORS - ENGINEERED SULFHYDRYL STUDIES. Biophysical Journal. 209-219. 1992
- QUANTITATING AND ENGINEERING THE ION SPECIFICITY OF AN EF-HAND-LIKE CA2+ BINDING-SITE. Biochemistry. 8690-8697. 1991
- OPEN CONFORMATION OF A SUBSTRATE-BINDING CLEFT - F-19 NMR-STUDIES OF CLEFT ANGLE IN THE D-GALACTOSE CHEMOSENSORY RECEPTOR. Biochemistry. 6484-6490. 1991
- F-19 NMR-STUDIES OF THE D-GALACTOSE CHEMOSENSORY RECEPTOR .1. SUGAR BINDING YIELDS A GLOBAL STRUCTURAL-CHANGE. Biochemistry. 4248-4256. 1991
- F-19 NMR-STUDIES OF THE D-GALACTOSE CHEMOSENSORY RECEPTOR .1. SUGAR BINDING YIELDS A GLOBAL STRUCTURAL-CHANGE. Biochemistry. 4248-4256. 1991
- F-19 NMR-STUDIES OF THE D-GALACTOSE CHEMOSENSORY RECEPTOR .2. CA(II) BINDING YIELDS A LOCAL STRUCTURAL-CHANGE. Biochemistry. 4257-4261. 1991
- F-19 NMR-STUDIES OF THE D-GALACTOSE CHEMOSENSORY RECEPTOR .2. CA(II) BINDING YIELDS A LOCAL STRUCTURAL-CHANGE. Biochemistry. 4257-4261. 1991
- CALCIUM(II) SITE SPECIFICITY - EFFECT OF SIZE AND CHARGE ON METAL-ION BINDING TO AN EF-HAND-LIKE SITE. Biochemistry. 3937-3943. 1990
- Global Flexibility in a Sensory Receptor: a Site-Directed Cross-Linking Approach. Science. 1596-1600. 1987
- Ion Channels within Ion Transport Proteins. Biophysical Journal. 91-92. 1984

Teaching

courses taught

BCHM 4400 - Core Concepts in Physical Chemistry for Biochemists
Primary Instructor - Spring 2020 / Spring 2022 / Spring 2024
Introduces thermodynamics, kinetics and spectroscopy, emphasizing macromolecule and biochemical applications. Includes thermodynamics, chemical and physical equilibriums, solution chemistry, rates of chemical and biochemical reactions, chemical bonds and principles and selected examples of spectroscopies applied to biological systems. Department enforced prerequisite or corequisite: PHYS 1120 or PHYS 2020. Formerly CHEM 4411. Same as BCHM 5400. Degree credit not granted for this course and CHEM 4511.
BCHM 4491 - Modern Biophysical Methods
Primary Instructor - Fall 2020 / Fall 2022 / Fall 2024
Covers the basic theory of biophysical methods widely employed in biochemistry and biology, including: electrophoresis, mass spec, calorimetry, evanescent waves, plasmon resonance, Xray diffraction, absorbance and fluorescence spectroscopy, magnetic resonance, electron and optical microscopy and single molecule methods. Discusses ways to maximize rigor and reproducibility in biophysical studies. Department enforced prerequisites: undergraduate chemistry (general, organic physical); physics; calculus. Recommended prerequisite or corequisite: BCHM 4400 or CHEM 4531. Same as BCHM 5491.
BCHM 5491 - Modern Biophysical Methods
Primary Instructor - Fall 2020 / Fall 2022 / Fall 2024
Covers the basic theory of biophysical methods widely employed in biochemistry and biology, including: electrophoresis, mass spec, calorimetry, evanescent waves, plasmon resonance, Xray diffraction, absorbance and fluorescence spectroscopy, magnetic resonance, electron and optical microscopy and single molecule methods. Discusses ways to maximize rigor and reproducibility in biophysical studies. Same as BCHM 4491. Department enforced prerequisites: undergraduate chemistry (general, organic, physical), biochemistry, physics, calculus.
BCHM 6601 - Biochemistry Seminar
Primary Instructor - Fall 2019 / Spring 2020 / Fall 2020 / Spring 2021 / Fall 2021 / Spring 2022 / Fall 2022 / Spring 2023 / Fall 2023 / Spring 2024
Restricted to and required for all currently funded NIH/CU Biophysics trainees and current NIH/CU Biophysics affiliates. Credit is deferred until presentation of satisfactory seminar. Formerly CHEM 6601.
BCHM 6901 - Research in Biochemistry
Primary Instructor - Fall 2022 / Fall 2023 / Spring 2024
May be repeated up to 15 total credit hours.
CHEM 6601 - Biochemistry Seminar
Primary Instructor - Spring 2018 / Fall 2018 / Spring 2019
Restricted to and required for all currently funded NIH/CU Biophysics trainees and current NIH/CU Biophysics affiliates. Credit is deferred until presentation of satisfactory seminar. Formerly CHEM 6601.
CHEM 6901 - Research in Chemistry
Primary Instructor - Spring 2018
May be repeated up to 15 total credit hours.
MCDB 6000 - Introduction to Laboratory Methods
Primary Instructor - Fall 2021 / Spring 2022
Introduces methodology and techniques used in biological research. Designed as a tutorial between a few students and one faculty member. Students are expected to read original research papers, discuss findings, and to plan and execute experiments in selected areas. May be repeated up to 15 total credit hours.

Background

education and training

Ph.D., California Institute of Technology 1984
B.A., Earlham College 1978

awards and honors

Outstanding Investigator Award (R35), conferred by National Institutes of Health, 2022
Fellow of the Biophysical Society, conferred by Biophysical Society, 2015

International Activities

geographic focus

http://dbpedia.org/page/Taiwan Geographic Region
Belgium Country
China Country
Europe Continent
Germany Country
Italy Country
United Kingdom Country

Other Profiles

ORCID iD

http://orcid.org/0000-0002-3704-4694

ResearchGate

https://www.researchgate.net/profile/Joseph_Falke

Academia.edu

https://www.colorado.edu/lab/falke/