My main research interests are functional and structural analyses by 3-D cryo-electron microscopy of cytoskeletal assemblies and macromolecular structures as well as cellular organelles. We apply cryo-electron tomography to investigate the structure function relationships of large macromolecular assemblies and cellular organelles within their native cytosolic environment. We operate two electron microscopes with the latest data recording systems, and peripheral equipment for specimen preparation and data analysis. I received funding to create a new Cryo-Electron Tomography facility (CU-Boulder Center for Cryo-ET: CCET, total funding about $8M). This will add a new FIBSEM microscope plus various peripheral equipment to the University and to MCD-Biology.
Cellbiology and Cytoskeleton assemblies, 3-D reconstruction of macromolecular assemblies and cellular organelles by cryo-electron microscopy, cryo-electron tomography
MCDB 3135 - Molecular Cell Biology I
Examines the central dogma of biology by discussing the most important molecules in cells (DNA, RNA and protein) and how their synthesis (DNA replication, transcription, RNA processing and translation) is regulated. Incorporated into the discussion is how recombinant DNA techniques are used to discover and dissect cellular processes, how to design and interpret experiments, and understanding the limits of experiments to draw conclusions.
MCDB 3501 - Structural Methods for Biological Macromolecules
Fall 2018 / Fall 2019 / Fall 2020 / Spring 2022
Teaches fundamental knowledge about protein structures, protein interactions and protein folding. Discusses in detail the most common methods on how proteins and macromolecular complexes are studied, such as X-ray crystallography, NMR-spectroscopy and electron microscopy. Offers about 50 percent direct teaching, 40 percent discussion of papers in a journal club style and 10 percent hands-on practicals on software packages relevant to structural biology. Recommended prerequisite: MCDB 2150 or EBIO 2070 (minimum grade C-). Formerly MCDB 4501.
MCDB 4100 - Special Topics
May be repeated up to 6 total credit hours. Recommended prerequisites: MCDB 3135 and MCDB 3145 (minimum grade C-).