A filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant.
Journal Article
Overview
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Adenocarcinoma - Carcinoma, Hepatocellular
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Animals
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Binding Sites
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Biochemical Phenomena - Kinetics
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Cell-Free System
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Chemical Phenomena - Filtration
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Chemistry Techniques, Analytical - Filtration
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Coenzymes - Tetrahydrofolates
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Cytoplasm
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Deoxyribonucleotides
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Digestive System Diseases - Carcinoma, Hepatocellular
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Digestive System Diseases - Liver Neoplasms
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Drug Resistance - Drug Resistance, Microbial
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Elements - Tritium
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Firmicutes - Lactobacillus casei
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Fluorine
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Folic Acid - Tetrahydrofolates
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Gases - Tritium
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Gram-Positive Asporogenous Rods, Regular - Lactobacillus casei
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Gram-Positive Bacteria - Lactobacillus casei
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Health Care Evaluation Mechanisms - Evaluation Studies as Topic
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Investigative Techniques - Evaluation Studies as Topic
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Liver Diseases - Carcinoma, Hepatocellular
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Liver Diseases - Liver Neoplasms
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Mechanical Phenomena - Kinetics
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Methods
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Methotrexate
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Methyltransferases
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Microbiological Phenomena - Drug Resistance, Microbial
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Models, Animal - Neoplasms, Experimental
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Neoplasms - Neoplasms, Experimental
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Neoplasms by Site - Carcinoma, Hepatocellular
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Neoplasms by Site - Liver Neoplasms
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Nucleic Acids, Nucleotides, and Nucleosides - Uracil Nucleotides
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Photometry - Spectrophotometry
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Physical Phenomena - Filtration
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Pyrimidines - Uracil Nucleotides
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Radioisotopes - Tritium
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Rats
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Ribonucleotides - Uracil Nucleotides
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Spectrum Analysis - Spectrophotometry
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Thymidylate Synthase
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Santi DV; McHenry CS; Perriard ER
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