Cloning and identification of the product of the dnaE gene of Escherichia coli. Journal Article uri icon



  • We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.

publication date

  • October 1, 1982

has subject area

has restriction

  • green

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Welch MM; McHenry CS

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9193

Additional Document Info

start page

  • 351

end page

  • 356


  • 152


  • 1