Single molecule studies of RNA polymerase II transcription in vitro. Journal Article uri icon



  • Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Elucidating the mechanism by which RNAP II synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. Significant advances in understanding RNAP II transcription have been achieved using classical biochemical and structural techniques; however, aspects of the transcription mechanism cannot be assessed using these approaches. The application of single-molecule techniques to study RNAP II transcription has provided new insight only obtainable by studying molecules in this complex system one at a time.

publication date

  • January 1, 2014

has subject area

has restriction

  • bronze

Date in CU Experts

  • March 18, 2015 5:22 AM

Full Author List

  • Horn AE; Goodrich JA; Kugel JF

author count

  • 3

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 2154-1272

Additional Document Info

start page

  • e27608


  • 5


  • 1