Nucleoside and nucleotide inactivation of R17 coat protein: evidence for a transient covalent RNA-protein bond. Journal Article uri icon



  • R17 coat protein forms a specific complex with a 21-nucleotide RNA hairpin containing the initiation site for the phage replicase gene. The RNA binding activity of the protein is inhibited by prior incubation with 5-bromouridine (BrU). The inactivation occurs with pseudo-first-order kinetics, and the inactive protein is stable to dilution. RNA binding activity of the BrU-inactivated protein is restored upon incubation with dithiothreitol. Inactivation of coat protein by N-ethylmaleimide or p-(chloromercuri)-benzenesulfonate indicates that a cysteine residue is located near the RNA binding site. Since 5-bromodeoxyuridine does not inactivate coat protein, a specific binding event appears to be required before inactivation can occur. Surprisingly, unmodified cytidine nucleotides also inactivate coat protein, with a specificity similar to the modified analogues. These results are discussed with regard to the formation of a transient covalent RNA-protein bond.

publication date

  • July 16, 1985

has subject area

has restriction

  • closed

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Romaniuk PJ; Uhlenbeck OC

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0006-2960

Additional Document Info

start page

  • 4239

end page

  • 4244


  • 24


  • 15