Methods in Molecular Biology
Overview
publication venue for
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Endocytosis Assays Using Cleavable Fluorescent Dyes
2022
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Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay
2022
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Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs.
2022
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Computational Modeling for Biomimetic Sensors
2019
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Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins
2019
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Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift
2019
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Quantification of Leaf Phloem Anatomical Features with Microscopy
2019
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Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically Encoded Biosensors
2019
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Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display
2018
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Dynamic Management of Codon Compression for Saturation Mutagenesis
2018
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Native Gel Approaches in Studying Proteasome Assembly and Chaperones
2018
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A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization
2017
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Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin
2017
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Calorimetric Measurement of SH2 Domain Ligand Affinities
2017
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Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
2017
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NMR Chemical Shift Mapping of SH2 Peptide Interactions
2017
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Transplantation of Skeletal Muscle Stem Cells
2017
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Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment
2016
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Isolation, Culture, Functional Assays, and Immunofluorescence of Myofiber-Associated Satellite Cells
2016
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Measuring TGF-β Ligand Dynamics in Culture Medium
2016
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Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data
2016
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Bioinformatics Analysis to Identify RNA-Protein Interactions in Oogenesis.
2015
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Efficient analysis methods in synthetic biology.
2015
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In vivo cross-linking followed by polyA enrichment to identify yeast mRNA binding proteins.
2015
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Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA
2015
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Studying the Affinity, Kinetic Stability, and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System
2015
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The synthetic biology open language.
2015
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Caspase Protocols in Caenorhabditis elegans
2014
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Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells Using Genetically Encoded Fluorescent Biosensors
2014
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An introduction to best practices in free energy calculations.
2013
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In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films.
2013
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Using FRET to monitor protein-induced DNA bending: the TBP-TATA complex as a model system.
2013
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Best Practices in Free Energy Calculations for Drug Design
2012
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Bistability in one equation or fewer
2012
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Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing
2012
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Single-molecule optical-trapping measurements with DNA anchored to an array of gold nanoposts.
2012
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Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain
2012
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LC/TOF-MS Analysis of Pesticides in Fruits and Vegetables: The Emerging Role of Accurate Mass in the Unambiguous Identification of Pesticides in Food
2011
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Screening for Radiation Sensitizers of Drosophila Checkpoint Mutants
2011
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Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces
2011
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Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF-beta Signaling
2010
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Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
2010
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Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting
2010
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Methods to Characterize the Effect of DNA-Modifying Compounds on Nucleosomal DNA
2010
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Single-Molecule Studies of RecBCD
2010
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Determining structures of RNA aptamers and riboswitches by X-ray crystallography.
2009
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Monitoring RNA-ligand interactions using isothermal titration calorimetry.
2009
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A simple crosslinking method, CLAMP, to map the sites of RNA-contacting domains within a protein.
2008
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Cardiomyocyte preparation, culture, and gene transfer.
2007
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Quantitative analysis of atherosclerotic lesion composition in mice.
2006
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Control of mitotic entry after DNA damage in Drosophila.
2004
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Methods in functional proteomics: two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients, in-gel digestion and identification of proteins by mass spectrometry.
2004
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Practical methods for deuterium exchange/mass spectrometry.
2004
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Two-dimensional gel electrophoresis for the identification of signaling targets.
2004
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Suppressor tRNAs: protocols and applications for cardiac gene transfer.
2003
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Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains.
2002
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Expression and purification of recombinant histones and nucleosome reconstitution.
1999
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Toeprinting assays. Mapping by blocks to reverse transcriptase primer extension.
1998
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Synthesis and purification of phosphorodithioate DNA.
1993
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Biomimetic Sensing
2019
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Computational Modeling of Signaling Networks
2012
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Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis
2022
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Forming Large Effective Ultrasound Arrays Using the Swept Synthetic Aperture Technique
2022
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Cross-Reactive, Self-Encoded Polymer Film Arrays for Sensor Applications
2019
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In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms
2019
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Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization
2019
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Midline (Central) Fluid Percussion Model of Traumatic Brain Injury
2016
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Visualization of IncRNA by Single-Molecule Fluorescence In Situ Hybridization
2015
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Seeded Microtubule Growth for Cryoelectron Microscopy of End-Binding Proteins
2014
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Preparation of Normalized cDNA Libraries for 454 Titanium Transcriptome Sequencing
2012
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Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
2011
Research
keywords
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0601 Biochemistry and Cell Biology, 0399 Other Chemical Sciences