Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs. Journal Article uri icon



  • Synthetic triterpenoids, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) and its derivatives are known to have potent anti-cancer and anti-inflammatory activities. The mechanisms of actions of CDDO and its derivatives as potential therapeutics remain elusive. Previous studies found that CDDO-Me triggers apoptosis by inducing extracellular Ca2+ influx followed by endoplasmic reticulum (ER)-derived vacuolation. Since Ca2+ activity in cells is dynamic and needs to be tracked in real time in living cells, we report a high-throughput and high-content imaging method to track CDDO-induced Ca2+ fluctuation in both ER and cytosol with MATLAB script for data analysis and visualization.

publication date

  • January 1, 2022

has restriction

  • closed

Date in CU Experts

  • February 23, 2023 11:26 AM

Full Author List

  • Liu Z; Ramirez A; Liu X

author count

  • 3

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Additional Document Info

start page

  • 227

end page

  • 236


  • 2488