PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells. Journal Article uri icon

Overview

abstract

  • Telomeric repeat-containing RNA (TERRA) molecules play important roles at telomeres, from heterochromatin regulation to telomerase activity control. In human cells, TERRA is transcribed from subtelomeric promoters located on most chromosome ends and associates with telomeres. The origin of mouse TERRA molecules is, however, unclear, as transcription from the pseudoautosomal PAR locus was recently suggested to account for the vast majority of TERRA in embryonic stem cells (ESC). Here, we confirm the production of TERRA from both the chromosome 18q telomere and the PAR locus in mouse embryonic fibroblasts, ESC, and various mouse cancer and immortalized cell lines, and we identify two novel sources of TERRA on mouse chromosome 2 and X. Using various approaches, we show that PAR-TERRA molecules account for the majority of TERRA transcripts, displaying an increase of two to four orders of magnitude compared to the telomeric 18q transcript. Finally, we present a SILAC-based pull-down screen revealing a large overlap between TERRA-interacting proteins in human and mouse cells, including PRC2 complex subunits, chromatin remodeling factors, DNA replication proteins, Aurora kinases, shelterin complex subunits, Bloom helicase, Coilin, and paraspeckle proteins. Hence, despite originating from distinct genomic regions, mouse and human TERRA are likely to play similar functions in cells.

publication date

  • January 1, 2021

has subject area

has restriction

  • bronze

Date in CU Experts

  • January 8, 2021 2:18 AM

Full Author List

  • Viceconte N; Loriot A; Lona Abreu P; Scheibe M; Fradera Sola A; Butter F; De Smet C; Azzalin CM; Arnoult N; Decottignies A

author count

  • 10

published in

  • RNA  Journal

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 1469-9001

Additional Document Info

start page

  • 106

end page

  • 121

volume

  • 27

issue

  • 1