abstract
- Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates. In the alternative area of organic chemical synthesis, significant effort has been channeled into improving coupling efficiencies and producing modified RNA analogs which retain activity but have higher stability than unmodified RNAs.