Binding of misacylated tRNAs to the ribosomal A site. Journal Article uri icon



  • To test whether the ribosome displays specificity for the esterified amino acid and the tRNA body of an aminoacyl-tRNA (aa-tRNA), the stabilities of 4 correctly acylated and 12 misacylated tRNAs in the ribosomal A site were determined. By introducing the GAC (valine) anticodon into each tRNA, a constant anticodon.codon interaction was maintained, thus removing concern that different anticodon.codon strengths might affect the binding of the different aa-tRNAs to the A site. Surprisingly, all 16 aa-tRNAs displayed similar dissociation rate constants from the A site. These results suggest that either the ribosome is not specific for different amino acids and tRNA bodies when intact aa-tRNAs are used or the specificity for the amino acid side chain and tRNA body is masked by a conformational change upon aa-tRNA release.

publication date

  • November 1, 2005

has subject area

has restriction

  • bronze

Date in CU Experts

  • March 13, 2015 12:56 PM

Full Author List

  • Dale T; Uhlenbeck OC

author count

  • 2

published in

  • RNA  Journal

Other Profiles

International Standard Serial Number (ISSN)

  • 1355-8382

Additional Document Info

start page

  • 1610

end page

  • 1615


  • 11


  • 11