Fluorometric assay for DNA polymerases and reverse transcriptase. Journal Article uri icon



  • We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with the conventional isotopic assay shows that the fluorometric assay accurately reflects the extent of replication. By performing the assay reactions directly in 96-well plates and using a fluorescence plate reader to determine the extent of reaction, the time required to measure replication activities is significantly shortened.

publication date

  • October 1, 1996

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Seville M; West AB; Cull MG; McHenry CS

author count

  • 4

citation count

  • 6

Other Profiles

International Standard Serial Number (ISSN)

  • 0736-6205

Electronic International Standard Serial Number (EISSN)

  • 1940-9818

Additional Document Info

start page

  • 664

end page

  • 672


  • 21


  • 4