The concepts of queer theory and heteronormativity have been taken up in educational research due to the influence of disciplines including gender and sexuality studies, feminist theory, and critical race theory. Queer theory seeks to disrupt dominant and normalizing binaries that structure our understandings of gender and sexuality. Heteronormativity describes the belief that heterosexuality is and should be the preferred system of sexuality and informs the related male or female, binary understanding of gender identity and expression. Taken together, queer theory and heteronormativity offer frames to interrogate and challenge systems of sex and gender in educational institutions and research to better support and understand the experiences of LGBTQ youth. They also inform the development of queer pedagogy that includes classroom and instructional practices designed to expand and affirm gender and sexual diversity in schools.