Covers the basic theory of biophysical methods widely employed in biochemistry and biology, including: electrophoresis, mass spec, calorimetry, evanescent waves, plasmon resonance, Xray diffraction, absorbance and fluorescence spectroscopy, magnetic resonance, electron and optical microscopy and single molecule methods. Discusses ways to maximize rigor and reproducibility in biophysical studies. Same as BCHM 4491. Department enforced prerequisites: undergraduate chemistry (general, organic, physical), biochemistry, physics, calculus.