The Design and Catalytic Properties of a Simplified Ribonuclease P RNA Journal Article uri icon



  • ; Ribonuclease P (RNase P) RNA is the catalytic moiety of the ribonucleoprotein enzyme that removes precursor sequences from the 5′ ends of pre-transfer RNAs in eubacteria. Phylogenetic variation according to recently proposed secondary structure models was used to identify structural elements of the RNase P RNA that are dispensable for catalysis. A simplified RNase P RNA that consists only of evolutionarily conserved features was designed, synthesized, and characterized. Although the simplified RNA (; Min; 1 RNA) is only 263 nucleotides in length, in contrast to the 354 to 417 nucleotides of naturally occurring RNase P RNAs, its specificity of pre-tRNA cleavage is identical to that of the native enzymes. Moreover, the catalytic efficiencies of the; Min; 1 RNA and the native RNA enzymes are similar. These results focus the search for the catalytic elements of RNase P RNAs to their conserved structure.;

publication date

  • June 30, 1989

has restriction

  • closed

Date in CU Experts

  • May 10, 2014 3:04 AM

Full Author List

  • Waugh DS; Green CJ; Pace NR

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0036-8075

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Additional Document Info

start page

  • 1569

end page

  • 1571


  • 244


  • 4912