Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells Journal Article uri icon



  • ; Rapid phylogenetic identification of single microbial cells was achieved with a new staining method. Formaldehyde-fixed, intact cells were hybridized with fluorescently labeled oligodeoxynucleotides complementary to 16; S; ribosomal RNA (rRNA) and viewed by fluorescence microscopy. Because of the abundance of rRNA in cells, the binding of the fluorescent probes to individual cells is readily visualized. Phylogenetic identification is achieved by the use of oligonucleotides (length 17 to 34 nucleotides) that are complementary to phylogenetic group-specific 16; S; rRNA sequences. Appropriate probes can be composed of oligonucleotide sequences that distinguish between the primary kingdoms (eukaryotes, eubacteria, archaebacteria) and between closely related organisms. The simultaneous use of multiple probes, labeled with different fluorescent dyes, allows the identification of different cell types in the same microscopic field. Quantitative microfluorimetry shows that the amount of an rRNA-specific probe that binds to; Escherichia coli; varies with the ribosome content and therefore reflects growth rate.;

publication date

  • March 10, 1989

has restriction

  • closed

Date in CU Experts

  • May 10, 2014 3:03 AM

Full Author List

  • DeLong EF; Wickham GS; Pace NR

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0036-8075

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Additional Document Info

start page

  • 1360

end page

  • 1363


  • 243


  • 4896