Role of the Protein Moiety of Ribonuclease P, a Ribonucleoprotein Enzyme Journal Article uri icon



  • ; The; Bacillus subtilis; ribonuclease P consists of a protein and an RNA. At high ionic strength the reaction is protein-independent; the RNA alone is capable of cleaving precursor transfer RNA, but the turnover is slow. Kinetic analyses show that high salt concentrations facilitate substrate binding in the absence of the protein, probably by decreasing the repulsion between the polyanionic enzyme and substrate RNAs, and also slow product release and enzyme turnover. It is proposed that the ribonuclease P protein, which is small and basic, provides a local pool of counter-ions that facilitates substrate binding without interfering with rapid product release.;

publication date

  • January 8, 1988

has restriction

  • closed

Date in CU Experts

  • May 10, 2014 1:32 AM

Full Author List

  • Reich C; Olsen GJ; Pace B; Pace NR

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0036-8075

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Additional Document Info

start page

  • 178

end page

  • 181


  • 239


  • 4836