Colour confocal reflection microscopy using red, green and blue lasers Journal Article uri icon



  • SUMMARYTo obtain colour reflected confocal images we have incorporated three lasers (HeNe: 633 nm; NdYAG: 532 nm; HeCd: 442 nm) and three photomultiplier detectors into our on‐axis scanning system then adjusted the registration of the simultaneous output signals to produce full‐colour images on a video monitor. Colour confocal images were produced from multi‐stained fixed tissue as well as from natural pigments in fresh plant material. Rayleigh scattering properties of immunogold‐labelled specimens were studied to show how variations in colour response can be utilized to identify subwavelength gold particles. Colour stereo pairs were produced to illustrate the accuracy with which the three‐laser microscope system can record depth information without incurring problems due to chromatic aberration effects.

publication date

  • January 1, 1992

has restriction

  • closed

Date in CU Experts

  • April 4, 2014 11:29 AM

Full Author List

  • Cogswell CJ; Hamilton DK; Sheppard CJR

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-2720

Electronic International Standard Serial Number (EISSN)

  • 1365-2818

Additional Document Info

start page

  • 103

end page

  • 117


  • 165


  • 1