Exosome-Mediated Recognition and Degradation of mRNAs Lacking a Termination Codon Journal Article uri icon

Overview

abstract

  • One role of messenger RNA (mRNA) degradation is to maintain the fidelity of gene expression by degrading aberrant transcripts. Recent results show that mRNAs without translation termination codons are unstable in eukaryotic cells. We used yeast mutants to demonstrate that these “nonstop” mRNAs are degraded by the exosome in a 3′-to-5′ direction. The degradation of nonstop transcripts requires the exosome-associated protein Ski7p. Ski7p is closely related to the translation elongation factor EF1A and the translation termination factor eRF3. This suggests that the recognition of nonstop mRNAs involves the binding of Ski7p to an empty aminoacyl-(RNA-binding) site (A site) on the ribosome, thereby bringing the exosome to a mRNA with a ribosome stalled near the 3′ end. This system efficiently degrades mRNAs that are prematurely polyadenylated within the coding region and prevents their expression.

publication date

  • March 22, 2002

has restriction

  • closed

Date in CU Experts

  • February 21, 2014 12:15 PM

Full Author List

  • van Hoof A; Frischmeyer PA; Dietz HC; Parker R

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0036-8075

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Additional Document Info

start page

  • 2262

end page

  • 2264

volume

  • 295

issue

  • 5563