Interactions between Brome Mosaic Virus RNAs and Cytoplasmic Processing Bodies Journal Article uri icon



  • ABSTRACT; ; Cytoplasmic processing bodies are sites where nontranslating mRNAs accumulate for different fates, including decapping and degradation, storage, or returning to translation. Previous work has also shown that the Lsm1-7p complex, Dhh1p, and Pat1p, which are all components of P bodies, are required for translation and subsequent recruitment to replication of the plant virus brome mosaic virus (BMV) genomic RNAs when replication is reproduced in yeast cells. To better understand the role of P bodies in BMV replication, we examined the subcellular locations of BMV RNAs in yeast cells. We observed that BMV genomic RNA2 and RNA3 accumulated in P bodies in a manner dependent on; cis; -acting RNA replication signals, which also directed nonviral RNAs to P bodies. Furthermore, the viral RNA-dependent RNA polymerase coimmunoprecipitates and shows partial colocalization with the P-body component Lsm1p. These observations suggest that the accumulation of BMV RNAs in P bodies may be an important step in RNA replication complex assembly for BMV, and possibly for other positive-strand RNA viruses.;

publication date

  • September 15, 2007

has restriction

  • green

Date in CU Experts

  • February 20, 2014 11:36 AM

Full Author List

  • Beckham CJ; Light HR; Amar Nissan T; Ahlquist P; Parker R; Noueiry A

author count

  • 6

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-538X

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Additional Document Info

start page

  • 9759

end page

  • 9768


  • 81


  • 18