5-Fluoro-2'-deoxyuridylate: covalent complex with thymidylate synthetase. Journal Article uri icon



  • 5-Fluoro-2'-deoxyuridylate causes a rapid inactivation of thymidylate synthetase that is dependent upon prior complexation of the cofactor 5,10-methylenetetrahydrofolate. The enzyme-5-fluoro-2'-deoxyuridylate complex may be isolated on nitrocellulose membranes and is not disrupted by 6 M urea. Upon reaction of 5-fluoro-2'-deoxyuridylate with the enzyme in the presence of 5,10-methylenetetrahydrofolate a rapid loss of absorbance is observed at 269 nm, the absorption maximum for the pyrimidine chromophore. It is concluded that a covalent bond is formed between the 6-position of 5-fluoro-2'-deoxyuridylate and a nucleophilic group of the enzyme that is involved in catalysis.

publication date

  • July 1, 1972

has subject area

has restriction

  • bronze

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Santi DV; McHenry CS

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0027-8424

Additional Document Info

start page

  • 1855

end page

  • 1857


  • 69


  • 7