Transcription and in vitro processing of yeast 5 S rRNA. Journal Article uri icon

Overview

abstract

  • A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5’-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3’-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.

publication date

  • January 1, 1980

Date in CU Experts

  • October 1, 2013 11:25 AM

Full Author List

  • Tekamp PA; Garcea RL; Rutter WJ

author count

  • 3

Additional Document Info

start page

  • 9501-6

end page

  • 9501-6

volume

  • 255

number

  • 19