Biophysical characterization of high-confidence, small human proteins. Journal Article uri icon

Overview

abstract

  • Significant efforts have been made to characterize the biophysical properties of proteins. Small proteins have received less attention because their annotation has historically been less reliable. However, recent improvements in sequencing, proteomics, and bioinformatics techniques have led to the high-confidence annotation of small open reading frames (smORFs) that encode for functional proteins, producing smORF-encoded proteins (SEPs). SEPs have been found to perform critical functions in several species, including humans. While significant efforts have been made to annotate SEPs, less attention has been given to the biophysical properties of these proteins. We characterized the distributions of predicted and curated biophysical properties, including sequence composition, structure, localization, function, and disease association of a conservative list of previously identified human SEPs. We found significant differences between SEPs and both larger proteins and control sets. In addition, we provide an example of how our characterization of biophysical properties can contribute to distinguishing protein-coding smORFs from noncoding ones in otherwise ambiguous cases.

publication date

  • September 11, 2024

has restriction

  • gold

Date in CU Experts

  • June 26, 2024 4:06 AM

Full Author List

  • Whited AM; Jungreis I; Allen J; Cleveland CL; Mudge JM; Kellis M; Rinn JL; Hough LE

author count

  • 8

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 2667-0747

Additional Document Info

start page

  • 100167

volume

  • 4

issue

  • 3