PhageBox: An Open Source Digital Microfluidic Extension with Applications for Phage Discovery Journal Article uri icon



  • <p>This work introduces an <em>open-source</em> hardware and software expansion for digital microfluidics (DMF) devices that integrates programmatic temperature and magnetic control. Our aim was to create a versatile, modular, extension that may aid in biomedical applications and has proven potential for use in bacteriophage research. To achieve this, we developed an <em>accessible </em>DMF device extension called PhageBox.</p>; <p>PhageBox incorporates temperature and magnetic control modules at the hardware level. At the software level, it is controlled by embedded software that includes a unique model for bio-protocol programming and a graphical user interface for visual feedback and operation of the device. We evaluated PhageBox's effectiveness in biomedical applications using functional testing and validated the temperature control using thermography, achieving a range of +/-0.2 Celsius. The electromagnets produced a magnetic force of 15 milliTesla, which precisely immobilized magnetic beads.</p>; <p>Furthermore, we demonstrate the potential of PhageBox in bacteriophage research through three initial proof of principle protocols: <u>(1)</u> a universal framework for PCR, <u>(2)</u> T7 bacteriophage restriction enzyme digestion, and <u>(3)</u> the concentration of PhiX174 RF genomic DNA. Overall, our study presents an open-source solution for DMF devices to integrate temperature regulation and magnetism, facilitating various applications in biomedical research and bacteriophage studies.</p>; <p>(<strong>GitHub:</strong> <a href="" target="_blank"></a>)</p>

publication date

  • March 27, 2023

has restriction

  • green

Date in CU Experts

  • April 5, 2023 8:37 AM

Full Author List

  • Albin D; Buecherl L; Kochavi E; Niehaus E; Novack S; Uragoda S; Myers CJ; Alistar M

author count

  • 8

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