Athermal, Chemically Triggered Release of RNA from Thioester Nucleic Acids Journal Article uri icon



  • AbstractAn athermal approach to mRNA enrichment from total RNA using a self‐immolative thioester linked nucleic acids (TENA) is described. Oligo(thymine) (oT) TENA has a six‐atom spacing between bases which allowed TENA to selectively base‐pair with polyadenine RNA. As a result of the neutral backbone of TENA and the hydrophobicity of the octanethiol end group, oT TENA is water insoluble and efficiently pulled down 93±2 % of EGFP mRNA at a concentration of 10 ng μL−1. Self‐immolative degradation of TENA upon ambient temperature exposure to nucleophilic buffer components (Tris, DTT) allowed recovery of 55±27 ng of mRNA from 3.1 μg of total RNA, which was not statistically different from the amount recovered using Dynabeads® mRNA DIRECT Kit (89±24 ng). Gene expression as measured by RT‐qPCR was comparable for both enrichment methods, suggesting that the mild conditions required for enrichment of mRNA using oT TENA are compatible with RT‐qPCR and other downstream molecular biology applications.

publication date

  • January 3, 2022

has restriction

  • bronze

Date in CU Experts

  • December 7, 2021 3:53 AM

Full Author List

  • Mavila S; Culver HR; Anderson AJ; Prieto TR; Bowman CN

author count

  • 5

Other Profiles

International Standard Serial Number (ISSN)

  • 0044-8249

Electronic International Standard Serial Number (EISSN)

  • 1521-3757

Additional Document Info


  • 134


  • 1