Dissociated hippocampal neurons exhibit distinct Zn; <sup>2+</sup>; dynamics in a stimulation method-dependent manner | CU Experts

ABSTRACT; ; Ionic Zn; 2+; has increasingly been recognized as an important neurotransmitter and signaling ion in glutamatergic neuron pathways. Intracellular Zn; 2+; transiently increases as a result of neuronal excitation, and this Zn; 2+; signal is essential for neuron plasticity, but the source and regulation of the signal is still unclear. In this study we rigorously quantified Zn; 2+; , Ca; 2+; and pH dynamics in dissociated mouse hippocampal neurons stimulated with bath application of high KCl or glutamate. While both stimulation methods yielded Zn; 2+; signals, Ca; 2+; influx, and acidification, glutamate stimulation induced more sustained high intracellular Ca; 2+; and a larger increase in intracellular Zn; 2+; . However, the stimulation-induced pH change was similar between conditions, indicating that a different cellular change is responsible for the stimulation-dependent difference in Zn; 2+; signal. This work provides the first robust quantification of Zn; 2+; dynamics in neurons using different methods of stimulation.;

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