Transcriptional profiling of the hyperthermophilic methanarchaeon; <i>Methanococcus jannaschii</i>; in response to lethal heat and non‐lethal cold shock | CU Experts

Summary; ; Temperature shock of the hyperthermophilic methanarchaeon; Methanococcus jannaschii; from its optimal growth temperature of 85°C to 65°C and 95°C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95°C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H; +; transporting ATP synthase. A gene encoding an α subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in; M. jannaschii; . Very different responses were observed upon cold shock to 65°C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP‐type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.;

Boonyaratanakornkit BB; Simpson AJ; Whitehead TA; Fraser CM; El‐Sayed NMA; Clark DS

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