Binding of yeast tRNAPhe anticodon arm to Escherichia coli 30 S ribosomes. Journal Article uri icon



  • A 15-nucleotide fragment of RNA having the sequence of the anticodon arm of yeast tRNAPhe was constructed using T4 RNA ligase. The stoichiometry and binding constant of this oligomer to poly(U)-programmed 30 S ribosomes was found to be identical to that of deacylated tRNAPhe. The anticodon arm and tRNAPhe also compete for the same binding site on the ribosome. These data indicate that the interaction of tRNAPhe with poly(U)-programmed 30 S ribosomes is primarily a result of contacts in the anticodon arm region and not with other parts of the transfer RNA. Since similar oligomers which cannot form a stable helical stem do not bind ribosomes, a clear requirement for the entire anticodon arm structure is demonstrated.

publication date

  • June 15, 1983

has restriction

  • closed

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Rose SJ; Lowary PT; Uhlenbeck OC

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-2836

Additional Document Info

start page

  • 103

end page

  • 117


  • 167


  • 1