A rapid method for localized mutagenesis of yeast genes Journal Article

Overview

abstract

• AbstractWe have developed a simple procedure for the localized mutagenesis of yeast genes. In this technique the region of interest is first amplified under mutagenic chain reaction (PCR) conditions. Cotransformation of the PCR product with a gapped plasmid containing homology to both ends the PCR procuct allows in vivo recombination to repair the gap with the mutagenized DNA. This procedure is efficient, allows trageting of specific regions for mutagenesis, and requires no subcloning steps in Escherichia coli.

CU Boulder Authors

• Parker, Roy

publication date

• February 1, 1992

has restriction

• closed

Date in CU Experts

• January 27, 2015 10:40 AM

Full Author List

• Muhlrad D; Hunter R; Parker R

author count

• 3

published in

• Yeast  Journal

Other Profiles

International Standard Serial Number (ISSN)

• 0749-503X

Electronic International Standard Serial Number (EISSN)

• 1097-0061

Digital Object Identifier (DOI)

• https://doi.org/10.1002/yea.320080202

Additional Document Info

start page

• 79

end page

• 82

volume

• 8

issue

• 2