Verifying the function and localization of genetically encoded Ca2+ sensors and converting FRET ratios to Ca2+ concentrations. Journal Article uri icon



  • Genetically encoded, ratiometric, fluorescent Ca(2+) biosensors can be used in living cells to quantitatively measure free Ca(2+) concentrations in the cytosol or in organelles. This protocol describes how to perform a calibration of a Ca(2+) sensor expressed in cultured mammalian cells as images are acquired using a widefield fluorescence microscope. This protocol also explains how to calculate Förster resonance energy transfer (FRET) ratios from acquired images and how to convert FRET ratios to Ca(2+) concentrations.

publication date

  • January 5, 2015

has subject area

has restriction

  • green

Date in CU Experts

  • January 26, 2015 11:17 AM

Full Author List

  • Park JG; Palmer AE

author count

  • 2

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 1559-6095

Additional Document Info

start page

  • pdb.prot076547


  • 2015


  • 1